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Annual Review of Medicine
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Introduction. Newly developed blood substitutes are based on two different proteins, hemoglobin and hemerythrin, respectively. In this paper we present the impact of the newly developed blood substitutes based on hemoglobin in Wistar rats and also we mention that this research is an explorative study. Hemoglobin-based blood substitutes are infusible oxygen-carrying liquids, obtained from bovine blood. This blood substitutes have no need for refrigeration or cross-matching, and are ideal for treating hemorrhagic shock in remote situations. Materials and methods. The experiment was performed in six Wistar male groups: Control, Native Hemoglobin, Polymerized Hemglobin, Copolymerized Hemoglobin with BSA, Copolymerized Hemoglobin with Rubrerythrin, Copolymerized Hemoglobin with Rubrerythrin-NROR and Polymerized Hemoglobin with o-ATP. All gropus received regular food and water ad libitum. The animals were healthy males, weighting 230±20 g and hosted in zoohygienical conditions into the zoobase of the Biological Research Institute from Cluj-Napoca. The blood substitutes were administrated via intravenous injection. The experiment lasted two days. The invitro tests were developed on human lymphocytes and their subpopulations also on the human vascular endothelial cells. Results and discussions. All the analyses that we have studied suggest a very good collaboration between the newly developed hemoglobin blood substitutes and in vivo and in vitro models used. The most important aspect of these tests is the fact that all blood substitutes do not produce any significant immunological reaction and also there is no any toxicity on human cell cultures. The biochemical and hematological tests do not suffer any significant changes. Conclusions. All the parameters that we have studied encourage us to continue this experiment we have already started. Acknowledgements to Florina Violeta Scurtu for the material support.
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Use of Organ Explant and Cell Culture in Cancer Research . Eric J. Kort, Christine R. Moore, Eric A. Hudson, Brandon Leeser, James H. Resau, GM Yerushalmi, R. Leibowitz-Amit, Galia Tsarfaty, Ilan Tsarfaty, and Sharon Moskovitz
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CA: A Cancer Journal for Clinicians
